Drosophila melanogaster miR-1012 stem-loop and Deep sequencing data

The plot graph shows the percentage of mismatches at each position from deep sequencing experiments. Different libraries are indicated in different colors.

Below the reference sequence, there are two modes of display using the same sequencing datasets: the Regular View and the Hybrid View. In the regular view, mismatches are highlighted in predefined colors (A-green; T-red; C-yellow; G-blue). In the hybrid view, all reads are condensed into a thin piece with a box below them indicating the total count of a particular read.

The last stripe is the stem-loop of dme-mir-1012. The light blue part stands for double strands stem and the light pink part is the single strand bulge. The light greens are representing for the gaps and the numbers on them are the length of the gaps. The light grey background shades mark the 5-prime and 3-prime region of the miRNA primary transcript.